Developing an olfactory cell therapy for spinal cord injury

Journal Publications

Mutso M, St John JA, Ling ZL, Burt FJ, Poo YS, Lio X, Žusinaite E, Grau GE, Hueston L, Merits A, King NJC, Ekberg J, Mahalingam S. Basic insights into Zika infection of neuroglial and brain endothelial cells. J Gen Virol, Apr 30 doi:10.1099/jgv.0.001416

Reshamwala R, Eindorf, T, Shah M, Smyth, G, Shelper T, St John J, Ekberg J. 2020 Induction of complete transection-type spinal cord injury in mice. J. Vis. Exp (159), accepted 24 March 2020,  doi: 10.3791/61131 .

Reshamwala R, Shah M, Belt L, Ekberg JAK, St John JA.  2020 Reliable cell purification and determination of cell purity: crucial aspects of olfactory ensheathing cell transplantation for spinal cord repair.  Neural Regen Res., 15(11):2016-2026,  doi: 10.4103/1673-5374.282218 

Surrao DC, Arasu Y, Ekberg JAK, St John JA. 2020. Blended, crosslinked alginate-methylcellulose hydrogels for encapsulation and delivery of olfactory ensheathing cells. Materialia 2020 May;10 doi:  10.1016/j.mtla.2020.100654 

Chen M, Vial ML, Gee L, Davis RA, St John J, Ekberg JAK. 2020.  The plant natural product 2-methoxy-1,4-naphthoquinone stimulates therapeutic neural repair properties of olfactory ensheathing cells  Sci. Rep. 10:951 doi: 10.1038/s41598-020-57793-2 

Gilmour A, Reshamwala R, Wright A, Ekberg J, St John J. 2020.  Optimizing olfactory ensheathing cell transplantation for spinal cord injury repair.  J Neurotrauma. 2020 Feb 14. doi: 10.1089/neu.2019.6939. 

Walkden H, Delbaz A, Nazareth L, Batzloff M, Shelper T, Beacham IR, Chacko A, Shah M, Beagley KW, Tello Velasquez J, St John JA, Ekberg JAK. 2020.  Burkholderia pseudomallei invades the olfactory nerve and bulb after epithelial injury in mice and causes the formation of multinucleated giant glial cells in vitro.  PLoS Negl Trop Dis  Jan 24;14(1):e0008017. doi: 10.1371/journal.pntd.0008017

Reshamwala R, Shah M, St John J, Ekberg J, 2020 The link between olfactory ensheathing cell survival and spinal cord injury repair: a commentary on common limitations of contemporary research. Neural Regeneration Research,  Oct;15(10):1848-1849. doi:  10.4103/1673-5374.280310 

Delbaz A, Chen M, Jen FE, Schulz BL, Gorse AD, Jennings MP, St John JA, Ekberg JAK. 2020.  Infect Immun.   Neisseria meningitidis induces pathology-associated cellular and molecular changes in trigeminal Schwann cells.  Jan 21. pii: IAI.00955-19  doi: 10.1128/IAI.00955-19. 

Wright AA, Todorovic M, Murtaza M, St John JA, Ekberg JA. 2020 Macrophage migration inhibitory factor and its binding partner HTRA1 are expressed by olfactory ensheathing cells. Jan;102:103450. doi: 10.1016/j.mcn.2019.103450  

Reshamwala R, Shah M, St John J, Ekberg J. 2019.  Survival and Integration of Transplanted Olfactory Ensheathing Cells are Crucial for Spinal Cord Injury Repair: Insights from the Last 10 Years of Animal Model Studies.  Cell Transplant Dec 28(1_suppl):132S-159S  doi: 10.1177/0963689719883823 

Chen, M., Vial, M. L., & St John, J. A. 2019. 3D-tips: user-friendly mesh barrier pipette tips for 3D-spheroid culture. Journal of biological engineering, 13, 80. doi:10.1186/s13036-019-0210-3   doi: 10.1186/s13036-019-0210-3. 

 Murtaza M, Chacko A, Delbaz A, Reshamwala R, Rayfield A, McMonagle B, St John JA, Ekberg JAK. 2019. Why are olfactory ensheathing cell tumors so rare?  Cancer Cell Int. 19, 260  doi: 10.1186/s12935-019-0989-5 

Beckingham LJ, Todorovic M, Tello Velasques J, Vial ML, Chen M, Ekberg J, St John JA. 2019 Three-dimensional cell culture can be regulated by vibration: low-frequency vibration increases the size of olfactory ensheathing cell spheroids.  J Biol Eng. 2019 May 16;13:41.  doi: 10.1186/s13036-019-0176-1 

Chen M, Shah MP, Shelper TB, Nazareth L, Barker M, Tello Velasquez J, Ekberg JAK, Vial ML, St John J. 2019. Naked liquid marbles: a robust three-dimensional low-volume cell culturing system. ACS Applied Materials & Interfaces.  Feb 6  doi: 10.1021/acsami.8b22036. 

Lum KY, Carroll AR, Ekins MG, Read S, Haq Z, Tietjen I, St John J, Davis RA. 2019. Capillasterin A, a novel pyronal[2,3-f]chromene from the Australian crinoid Capillaster multiradiatus. Marine Drugs 17, 26   doi: 10.3390/md17010026 

Nazareth L, Chen M, Shelper T, Shah M, Tello Velasquez J, Walkden H, Beacham I, Batzloff M, Rayfield A, Todorovic M, Beagley KW, St John JA, Ekberg JAK. 2019. Novel insights into the glia limitans of the olfactory nervous system. J Comp Neurol.   doi: 10.1002/cne.24618 

Chen M, Vial ML, Tello Velasquez J, Ekberg JA, Davis RA, St John JA. 2018. The serrulatane diterpenoid natural products RAD288 and RAD289 stimulate properties of olfactory ensheathing cells useful for neural repair therapies.  Scientific Reports, Jul 6;8(1):10240. doi: 10.1038/s41598-018-28551-2 

Beecher K, Hafner LM, Ekberg J, St John JA, Chehrehasa F. 2018 Combined VEGF/PDGF improves olfactory regeneration after unilateral bulbectomy in mice.   Neural Regen Res. 2018 Oct;13(10):1820-1826.   doi: 10.4103/1673-5374.238713 

Yao R, Murtaza M, Velasquez JT, Todorovic M, Rayfield A, Ekberg J, Barton M, St John J. Olfactory Ensheathing Cells for Spinal Cord Injury: Sniffing Out the Issues. Cell Transplant. 2018;27(6):879-889.

Wright AA, Todorovic M, Tello-Velasquez J, Rayfield AJ, St John JA, Ekberg JA. Enhancing the Therapeutic Potential of Olfactory Ensheathing Cells in Spinal Cord Repair Using Neurotrophins. Cell Transplant. 2018;27(6):867-878.

Chehrehasa F, Jacques A, St John JA*, Ekberg JA*. 2018. The Grueneberg olfactory organ neuroepithelium recovers after injury.  Brain Res. 2018 Jun 1;1688:65-72. doi: 10.1016/j.brainres.2018.03.020.  

Tello Velasquez J, St John JA, Nazareth L, Ekberg JA. 2018. Schwann cell lamellipodia regulate cell-cell interactions and phagocytosis. Molecular and Cellular Neuroscience, 88: 189-200.  doi: 10.1016/j.mcn.2018.01.001  

Rahim S, St John JA, Crane D, Meedeniya A. 2018. Impaired neurogenesis and associated gliosis in mouse brain with PEX13 deficiency.  Mol Cell Neurosci. 2018 Apr;88:16-32.   doi: 10.1016/j.mcn.2017.11.015 

Beecher K, St John JA, Chehrehasa F. 2018. Factors that modulate olfactory dysfuntion: a review. Accepted for publication in Neural Regeneration Research 7 May 2018, NRR-D-18-00123.

Kim T, Sirdaarta J, Zhang Q, Eftekhari E, St John J, Kennedy D, Cock I, Li Q. 2018. Selective Toxicity of Hydroxyl-Rich Carbon Nanodots for Cancer Research. Nano Research.  (2018) 11: 2204.    

Barton M, St John J, Clarke M, Wright A, Ekberg J. 2017. The glia response after peripheral nerve injury: a comparison between Schwann cells and olfactory ensheathing cells. International Journal of Molecular Sciences, 18:287, doi:10.3390/ijms18020287.

Kim T, White A, Sirdaarta J, Ji W, Cock I, St John J, Boyd S, Brown C, Li Q. Yellow-Emitting Carbon Nanodots and their Flexible and Transparent Films for White LEDs.   ACS Appl. Mater. Interfaces 2016, 8, 48 33102-33111 

Munaz A, Vadivelu RK, St John JA, Nguyen NT. 2016. A lab-on-a-chip device for investigating the fusion process of olfactory ensheathing cell spheroids.  Lab Chip. 2016 Aug 7;16(15):2946-54. .    

St John JA. 2016. Trojan horse L-selectin monocytes: a portal of Burkholderia pseudomallei entry into the brain. Virulence, Oct 20:1-2.  doi: 10.1080/21505594.2016.1250997 

Munaz A, Nguyen NT, Vadivelu RK, St John JA, Barton M, Kamble H. 2016. Three-dimensional printing of biological matters. J Sci Adv Mat Dev. Mar 1(1): 1-17

 St John JA, Walkden H, Nazareth L, Beagley KW, Ulett GC, Batzloff M, Beacham IR, Ekberg JAK. 2016. Burkholderia pseudomallei rapidly infects the brainstem and spinal cord via the trigeminal nerve after intranasal inoculation. Immunity and Infection, 84:2681-8.  

Tello Velasquez J, Yao RQ, Lim F, Han C, Ojika M, Ekberg JAK, Quinn RJ, St John JA. 2016. Linckosides enhance proliferation and induce morphological changes in human olfactory ensheathing cells. Molecular and Cellular Neuroscience, 75:1-13.

Harshad K, Jun M, Park S, Barton MJ, Vadivelu RK, St John J, Nguyen NT. 2016. An electromagnetic cell-stretching device for mechanotransduction studies of olfactory ensheathing cells. Biomedical Microdevices, 18(3):45.  doi: 10.1007/s10544-016-0071-1. 

Barton M, St John JA, Tatian A, Riches JD, Mograby O, Mahns DA. 2016. Morphological and morphometric analysis of the distal branches of the rat brachial plexus. Italian Journal of Anatomy and Embryology, 121: 240-252. 

Khaw MK, Ooi CH, Mohd-Yasin F, Vadivelu R, St John JA, Nguyen NT. 2016. Digital microfluidics with magnetically actuated floating liquid marble. Lab on a Chip, 16(12):2211-8.  doi: 10.1039/c6lc00378h 

Dando S, Ipe D, Batzloff M, Sullivan M, Crossman D, Crowley M, Strong E, Kyan S, Leclercq S, Ekberg J, St John J, Beachan I, Ulett G. 2016. Burkholderia pseudomallei capsule exacerabates respiratory melioidosis but does not afford protection against antimicrobial signalling or bacterial killing in human olfactory ensheathing cells. Infection and Immunity, 84(7):1941-56.  doi: 10.1128/IAI.01546-15 

Tello Velasquez J, Nazareth L, Quinn RJ, Ekberg JAK, St John JA. 2016. Stimulating the proliferation, migration and lamellipodia of Schwann cells using low-dose curcumin. Neuroscience, 324:140-150.

Ooi CH, Plackowski C, Nguyen AV, Vadivelu RK, St John JA, Dao DV, Nguyen NT. 2016. Floating mechanism of a small liquid marble. Scientific Reports, 6:21777.  doi: 10.1038/srep21777 

Vadivelu RK, Ooi CH, Yao RQ, Tello Velasques J, Pastrana E, Diaz-Nido J, Lim F, Ekberg JAK, Nguyen NT, St John JA. 2015. Generation of three-dimensional multiple spheroid model of olfactory ensheathing cells using floating liquid marbles. Scientific Reports, 5:15083.

Nazareth L, Tello Velasquez J, Lineburg KE, Chehrehasa F, St John JA*, Ekberg JAK*. 2015. Differing phagocytic capacities of accessory and main olfactory ensheathing cells and the implication for olfactory glia transplantation therapies. Molecular Cellular Neuroscience, 65:92-101.

Ooi CH, Vadivelu R, St John JA, Dao DV, Nguyen NT. 2015. Deformation of a floating liquid marble. Soft Matter, 11: 4576-4583  doi: 10.1039/c4sm02882a. 

Nazareth L, Lineburg KE, Chuah MI, Tello Velasquez J, Chehrehasa F, St John JA*,Ekberg JAK*. 2015. Olfactory ensheathing cells are the main phagocytic cells that remove axon debris during early development of the olfactory system. J Comp Neurol, 523:479-494 

Ekberg JAK, St John JA. 2015. Olfactory ensheathing cells for spinal cord repair: crucial differences between subpopulations of the glia. Invited review for Neural Regeneration Research, 10: 1395-1396.  doi: 10.4103/1673-5374 

Amaya DA, Ekberg JAK, St John JA. 2015. Radial glia interact with primary olfactory axons to regulate development of the olfactory bulb. Invited review for Neural Regeneration Research, 10(3): 374-376. 

Dando SJ, Mackay-Sim A, Norton R, Currie BJ, St John JA, Ekberg JAK, Batzloff M, Ulett GC, Beacham IR. 2014. Pathogens penetrating the central nervous system: infection pathways and the cellular and molecular mechanisms of invasion. Clinical Microbiology Reviews, 27:691-726.  

Ekberg JAK, St John JA. 2014. Crucial roles for olfactory ensheathing cells and olfactory mucosal cells in the repair of damaged neural tracts. Invited Review for Anatomical Record, 297:121-128.  

Tello Velasquez J, Watts ME, Todorovic M, Nazareth L, Pastrana E, Diaz-Nido J, Lim F, Ekberg JAK, Quinn RJ, St John JA. 2014. Low-dose curcumin stimulates the proliferation, migration and phagocytic activity of olfactory ensheathing cells. PLoS ONE, 9:e111787.  

St John JA, Ekberg JAK, Dando S, Meedeniya ACB, Horton RE, Batzloff M, Owen SJ, Holt S, Peak IR, Ulett G, Mackay-Sim A, Beacham IR. 2014. Burkholderia pseudomallei penetrates the brain via destruction of the olfactory and trigeminal nerves: implications for the pathogenesis of neurological melioidosis. mBIO, 5(2):e00025,   

 Panni P, Ferguson I, Beacham I, Mackay-Sim A, Ekberg JAK, St John JA. 2013. Phagocytosis of bacteria by olfactory ensheathing cells and Schwann cells. Neuroscience Letters, 539: 65-70  doi:  10.1016/j.neulet.2013.01.052 

 Thyssen A, Stavermann M, Buddruss K, Doengi M, Ekberg JA, St John JA, Deitmer JW, Lohr C. 2013. Spatial and developmental heterogeneity of calcium signaling in olfactory ensheathing cells. Glia, 61: 327-337.  

 Stavermann M, Buddrus K, St John JA, Ekberg JAK, Nilius B, Deitmer JW, Lohr C. 2012. Temperature-dependent calcium-induced calcium release via InsP3 receptors in olfactory ensheathing glial cells. Cell Calcium, 52: 113-123.  

 Ekberg JAK, Amaya D, Mackay-Sim A, St John JA. 2012. The migration of olfactory ensheathing cells during development and regeneration. Invited review for NeuroSignals, 20: 147-158.  

 Chehrehasa F, Ekberg JAK, Lineburg KE, Amaya D, Mackay-Sim A, St John JA. 2012. Two phases of replacement replenish the olfactory ensheathing cell population after injury in postnatal mice. Glia, 60: 322-332. 

 Windus LCE, Chehrehasa F, Lineburg K, Claxton C, Mackay-Sim A, St John JA. 2011. Stimulation of olfactory ensheathing cell motility enhances olfactory axon growth. Cellular and Molecular Life Sciences, 68: 3233-3247 

Ekberg JAK, Amaya D, Chehrehasa F, Lineburg K, Claxton C, Windus LCE, Key B, Mackay-Sim A, St John JA. 2011. OMP-ZsGreen fluorescent protein transgenic mice for visualisation of olfactory sensory neurons in vivo and in vitro. Journal of Neuroscience Methods, 196:88-98. 

Ekberg JA, St John JA. 2011. Repairing damaged brains and spinal cords: crucial roles for olfactory ensheathing cells. ChemoSense, 13: 1-9.

Mackay-Sim A, St John JA. 2011. Olfactory ensheathing cells from the nose: clinical application in human spinal cord injuries. Experimental Neurology, 229: 174-180. 

Windus CE, Lineburg KE, Scott SE, Claxton C, Mackay-Sim A, Key B, St John JA. 2010. Lamellipodia mediate the heterogeneity of central olfactory ensheathing cell interactions. Cellular and Molecular Life Sciences, 67:1735-50. 

 Windus LCE, Claxton C, Allen CL, Key B, St John JA. 2007. Motile membrane protrusions regulate cell-cell adhesion and migration of olfactory ensheathing glia. Glia, 55:1708-19. 

BOOK Chapters

Mackay-Sim A, St John JA, Schwob JE. 2015. Chapter 7: Neurogenesis in the adult olfactory epithelium. In: Handbook of Olfaction and Gustation, 3rd edition, pp133-156; edited by RL Doty, John Wiley & Sons Inc.

Tello Velasquez JT, Ekberg JAK, St John JA. 2015. Transplantation of olfactory ensheathing cells in spinal cord injury. Chapter 13: pp 277-309. In: Cellular Therapy for Stoke and CNS Injuries, edited by Li-Ru Zhao, John H Zhang, Springer, ISBN 978-3-319-11481-1