Developing an olfactory cell therapy for spinal cord injury

Journal Publications

Chen, M., Vial, M. L., & St John, J. A. (2019). 3D-tips: user-friendly mesh barrier pipette tips for 3D-spheroid culture. Journal of biological engineering, 13, 80. doi:10.1186/s13036-019-0210-3 

 Murtaza M, Chacko A, Delbaz A, Reshamwala R, Rayfield A, McMonagle B, St John JA, Ekberg JAK. 2019 Why are olfactory ensheathing cell tumors so rare?  Cancer Cell Int. 19, 260 

Beckingham LJ, Todorovic M, Tello Velasques J, Vial ML, Chen M, Ekberg J, St John JA. 2019 Three-dimensional cell culture can be regulated by vibration: low-frequency vibration increases the size of olfactory ensheathing cell spheroids.  J Biol Eng. 2019 May 16;13:41. 

Chen M, Shah MP, Shelper TB, Nazareth L, Barker M, Tello Velasquez J, Ekberg JAK, Vial ML, St John J. 2019. Naked liquid marbles: a robust three-dimensional low-volume cell culturing system. ACS Applied Materials & Interfaces.  Feb 6 

Lum KY, Carroll AR, Ekins MG, Read S, Haq Z, Tietjen I, St John J, Davis RA. 2019. Capillasterin A, a novel pyronal[2,3-f]chromene from the Australian crinoid Capillaster multiradiatus. Marine Drugs 17, 26 

Nazareth L, Chen M, Shelper T, Shah M, Tello Velasquez J, Walkden H, Beacham I, Batzloff M, Rayfield A, Todorovic M, Beagley KW, St John JA, Ekberg JAK. 2019. Novel insights into the glia limitans of the olfactory nervous system. J Comp Neurol.  

Chen M, Vial ML, Tello Velasquez J, Ekberg JA, Davis RA, St John JA. 2018. The serrulatane diterpenoid natural products RAD288 and RAD289 stimulate properties of olfactory ensheathing cells useful for neural repair therapies. Accepted for publication in Scientific Reports, 20 June 2018, SREP-17-32737B.

Beecher K, Hafner LM, Ekberg J, St John JA, Chehrehasa F. 2018 Combined VEGF/PDGF improves olfactory regeneration after unilateral bulbectomy in mice.   Neural Regen Res. 2018 Oct;13(10):1820-1826. 

Yao R, Murtaza M, Velasquez JT, Todorovic M, Rayfield A, Ekberg J, Barton M, St John J. Olfactory Ensheathing Cells for Spinal Cord Injury: Sniffing Out the Issues. Cell Transplant. 2018;27(6):879-889.

Wright AA, Todorovic M, Tello-Velasquez J, Rayfield AJ, St John JA, Ekberg JA. Enhancing the Therapeutic Potential of Olfactory Ensheathing Cells in Spinal Cord Repair Using Neurotrophins. Cell Transplant. 2018;27(6):867-878.

Chehrehasa F, Jacques A, St John JA*, Ekberg JA*. 2018. The Grueneberg olfactory organ neuroepithelium recovers after injury.  Brain Res. 2018 Jun 1;1688:65-72. doi: 10.1016/j.brainres.2018.03.020.  

Tello Velasquez J, St John JA, Nazareth L, Ekberg JA. 2018. Schwann cell lamellipodia regulate cell-cell interactions and phagocytosis. Molecular and Cellular Neuroscience, 88: 189-200.  

Rahim S, St John JA, Crane D, Meedeniya A. 2018. Impaired neurogenesis and associated gliosis in mouse brain with PEX13 deficiency.  Mol Cell Neurosci. 2018 Apr;88:16-32.  

Beecher K, St John JA, Chehrehasa F. 2018. Factors that modulate olfactory dysfuntion: a review. Accepted for publication in Neural Regeneration Research 7 May 2018, NRR-D-18-00123.

Kim T, Sirdaarta J, Zhang Q, Eftekhari E, St John J, Kennedy D, Cock I, Li Q. 2018. Selective Toxicity of Hydroxyl-Rich Carbon Nanodots for Cancer Research. Nano Research.  (2018) 11: 2204.    

Barton M, St John J, Clarke M, Wright A, Ekberg J. 2017. The glia response after peripheral nerve injury: a comparison between Schwann cells and olfactory ensheathing cells. International Journal of Molecular Sciences, 18:287, doi:10.3390/ijms18020287.

Kim T, White A, Sirdaarta J, Ji W, Cock I, St John J, Boyd S, Brown C, Li Q. Yellow-Emitting Carbon Nanodots and their Flexible and Transparent Films for White LEDs.   ACS Appl. Mater. Interfaces 2016, 8, 48 33102-33111 

Munaz A, Vadivelu RK, St John JA, Nguyen NT. 2016. A lab-on-a-chip device for investigating the fusion process of olfactory ensheathing cell spheroids.  Lab Chip. 2016 Aug 7;16(15):2946-54. .    

Munaz A, Nguyen NT, Vadivelu RK, St John JA, Barton M, Kamble H. 2016. Three-dimensional printing of biological matters. J Sci Adv Mat Dev. Mar 1(1): 1-17

 St John JA, Walkden H, Nazareth L, Beagley KW, Ulett GC, Batzloff M, Beacham IR, Ekberg JAK. 2016. Burkholderia pseudomallei rapidly infects the brainstem and spinal cord via the trigeminal nerve after intranasal inoculation. Immunity and Infection, 84:2681-8.  

Tello Velasquez J, Yao RQ, Lim F, Han C, Ojika M, Ekberg JAK, Quinn RJ, St John JA. 2016. Linckosides enhance proliferation and induce morphological changes in human olfactory ensheathing cells. Molecular and Cellular Neuroscience, 75:1-13.

Harshad K, Jun M, Park S, Barton MJ, Vadivelu RK, St John J, Nguyen NT. 2016. An electromagnetic cell-stretching device for mechanotransduction studies of olfactory ensheathing cells. Biomedical Microdevices, 18(3):45.

Barton M, St John JA, Tatian A, Riches JD, Mograby O, Mahns DA. 2016. Morphological and morphometric analysis of the distal branches of the rat brachial plexus. Italian Journal of Anatomy and Embryology, 121: 240-252. 

Khaw MK, Ooi CH, Mohd-Yasin F, Vadivelu R, St John JA, Nguyen NT. 2016. Digital microfluidics with magnetically actuated floating liquid marble. Lab on a Chip, 16(12):2211-8.

Dando S, Ipe D, Batzloff M, Sullivan M, Crossman D, Crowley M, Strong E, Kyan S, Leclercq S, Ekberg J, St John J, Beachan I, Ulett G. 2016. Burkholderia pseudomallei capsule exacerabates respiratory melioidosis but does not afford protection against antimicrobial signalling or bacterial killing in human olfactory ensheathing cells. Infection and Immunity, 84(7):1941-56.

Tello Velasquez J, Nazareth L, Quinn RJ, Ekberg JAK, St John JA. 2016. Stimulating the proliferation, migration and lamellipodia of Schwann cells using low-dose curcumin. Neuroscience, 324:140-150.

Ooi CH, Plackowski C, Nguyen AV, Vadivelu RK, St John JA, Dao DV, Nguyen NT. 2016. Floating mechanism of a small liquid marble. Scientific Reports, 6:21777.

Vadivelu RK, Ooi CH, Yao RQ, Tello Velasques J, Pastrana E, Diaz-Nido J, Lim F, Ekberg JAK, Nguyen NT, St John JA. 2015. Generation of three-dimensional multiple spheroid model of olfactory ensheathing cells using floating liquid marbles. Scientific Reports, 5:15083.

Nazareth L, Tello Velasquez J, Lineburg KE, Chehrehasa F, St John JA*, Ekberg JAK*. 2015. Differing phagocytic capacities of accessory and main olfactory ensheathing cells and the implication for olfactory glia transplantation therapies. Molecular Cellular Neuroscience, 65:92-101.

Ooi CH, Vadivelu R, St John JA, Dao DV, Nguyen NT. 2015. Deformation of a floating liquid marble. Soft Matter, 11: 4576-4583 

Nazareth L, Lineburg KE, Chuah MI, Tello Velasquez J, Chehrehasa F, St John JA*,Ekberg JAK*. 2015. Olfactory ensheathing cells are the main phagocytic cells that remove axon debris during early development of the olfactory system. J Comp Neurol, 523:479-494 

Ekberg JAK, St John JA. 2015. Olfactory ensheathing cells for spinal cord repair: crucial differences between subpopulations of the glia. Invited review for Neural Regeneration Research, 10: 1395-1396. 

Amaya DA, Ekberg JAK, St John JA. 2015. Radial glia interact with primary olfactory axons to regulate development of the olfactory bulb. Invited review for Neural Regeneration Research, 10(3): 374-376. 

Dando SJ, Mackay-Sim A, Norton R, Currie BJ, St John JA, Ekberg JAK, Batzloff M, Ulett GC, Beacham IR. 2014. Pathogens penetrating the central nervous system: infection pathways and the cellular and molecular mechanisms of invasion. Clinical Microbiology Reviews, 27:691-726.  

Ekberg JAK, St John JA. 2014. Crucial roles for olfactory ensheathing cells and olfactory mucosal cells in the repair of damaged neural tracts. Invited Review for Anatomical Record, 297:121-128.  

Tello Velasquez J, Watts ME, Todorovic M, Nazareth L, Pastrana E, Diaz-Nido J, Lim F, Ekberg JAK, Quinn RJ, St John JA. 2014. Low-dose curcumin stimulates the proliferation, migration and phagocytic activity of olfactory ensheathing cells. PLoS ONE, 9:e111787.  

St John JA, Ekberg JAK, Dando S, Meedeniya ACB, Horton RE, Batzloff M, Owen SJ, Holt S, Peak IR, Ulett G, Mackay-Sim A, Beacham IR. 2014. Burkholderia pseudomallei penetrates the brain via destruction of the olfactory and trigeminal nerves: implications for the pathogenesis of neurological melioidosis. mBIO, 5(2):e00025,   

 Thyssen A, Stavermann M, Buddruss K, Doengi M, Ekberg JA, St John JA, Deitmer JW, Lohr C. 2013. Spatial and developmental heterogeneity of calcium signaling in olfactory ensheathing cells. Glia, 61: 327-337.  

 Stavermann M, Buddrus K, St John JA, Ekberg JAK, Nilius B, Deitmer JW, Lohr C. 2012. Temperature-dependent calcium-induced calcium release via InsP3 receptors in olfactory ensheathing glial cells. Cell Calcium, 52: 113-123.  

 Ekberg JAK, Amaya D, Mackay-Sim A, St John JA. 2012. The migration of olfactory ensheathing cells during development and regeneration. Invited review for NeuroSignals, 20: 147-158.  

 Chehrehasa F, Ekberg JAK, Lineburg KE, Amaya D, Mackay-Sim A, St John JA. 2012. Two phases of replacement replenish the olfactory ensheathing cell population after injury in postnatal mice. Glia, 60: 322-332. 

 Windus LCE, Chehrehasa F, Lineburg K, Claxton C, Mackay-Sim A, St John JA. 2011. Stimulation of olfactory ensheathing cell motility enhances olfactory axon growth. Cellular and Molecular Life Sciences, 68: 3233-3247 

Ekberg JAK, Amaya D, Chehrehasa F, Lineburg K, Claxton C, Windus LCE, Key B, Mackay-Sim A, St John JA. 2011. OMP-ZsGreen fluorescent protein transgenic mice for visualisation of olfactory sensory neurons in vivo and in vitro. Journal of Neuroscience Methods, 196:88-98. 

Ekberg JA, St John JA. 2011. Repairing damaged brains and spinal cords: crucial roles for olfactory ensheathing cells. ChemoSense, 13: 1-9.

Mackay-Sim A, St John JA. 2011. Olfactory ensheathing cells from the nose: clinical application in human spinal cord injuries. Experimental Neurology, 229: 174-180. 

Windus CE, Lineburg KE, Scott SE, Claxton C, Mackay-Sim A, Key B, St John JA. 2010. Lamellipodia mediate the heterogeneity of central olfactory ensheathing cell interactions. Cellular and Molecular Life Sciences, 67:1735-50. 

 Windus LCE, Claxton C, Allen CL, Key B, St John JA. 2007. Motile membrane protrusions regulate cell-cell adhesion and migration of olfactory ensheathing glia. Glia, 55:1708-19. 

BOOK Chapters

Mackay-Sim A, St John JA, Schwob JE. 2015. Chapter 7: Neurogenesis in the adult olfactory epithelium. In: Handbook of Olfaction and Gustation, 3rd edition, pp133-156; edited by RL Doty, John Wiley & Sons Inc.

Tello Velasquez JT, Ekberg JAK, St John JA. 2015. Transplantation of olfactory ensheathing cells in spinal cord injury. Chapter 13: pp 277-309. In: Cellular Therapy for Stoke and CNS Injuries, edited by Li-Ru Zhao, John H Zhang, Springer, ISBN 978-3-319-11481-1